Fig. 3.
CM of Gal-3–overexpressing cells contains more growth-promoting soluble factors. MYL cells (A) or HS-5 cells (B) were grown with mixtures of various concentrations of CM/MYL and CM/MYL/G3. MYL cells (C) or HS-5 cells (D) were grown in complete medium containing various concentrations of rhGal-3 for 96 h. Cell proliferation was determined by means of methyl-thiazol-diphenyl-tetrazolium (MTT) assay. An increasing in concentration of CM/MYL/G3 promoted the cell proliferation of MYL cells and HS-5 cells, whereas the addition of rhGal-3 up to 10 ng/mL did not.