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. 2011 Oct 10;108(42):17275-17280. doi: 10.1073/pnas.1114255108

Fig. 5.

Fig. 5.

The ability of phenyl glycolipids to stimulate cytokine productions of human naïve Vα24 iNKT cells. Human naïve Vα24 iNKT cells (1 × 105) were cultured with autologous immature CD14+ dendritic cells (5 × 104) pulsed with the indicated glycolipids at 100 ng/mL for 18 h. The production of IFN-γ (A), IL-4 (B) and IL-10 (D) were detected by Beadlyte Human Cytokine Kit and read by a Luminex100 system. The ratio of IFN-γ over IL-4 (C) and IFN-γ over IL-10 (D), normalized to DMSO, were calculated. Assays were performed in triplicates and data were presented as mean ± SD. The symbol * represents p < 0.05, compared with DMSO; and #, p < 0.05, compared with C1.