Table 2. Thermodynamic parameters derived from calorimetric titrations of ATP and ADP nucleotides to Af-GlnK3.

Ligand	GlnK3	Temp. (°C)	No. binding sites per trimer	Association constantKA (M−1)	Dissociation constantKD (µM)	EntalphyΔH(cal·mol−1)	EntropyΔS(cal·mol−1·K−1)	Gibbs Free EnergyΔG (cal·mol−1)
MgATP	Wild-type	30	2.6±0.2	2960±250	338	−7078±799	−7.5	−4804
		70	3	763±42	1311	−4955±147	−1.2	−4543
	F86I	30	3.0±0.2	6690±824	149	−1115±85	13.8	−5298
	F86P	30	2.87±0.04	7840±307	128	−12000±242	−21.8	−5391
MgADP	Wild-type	30	2.8±0.2	1760±89	568	−3538±305	3.2	−4508
		70	3	413±13	2421	−4379±91	−0.8	−4104
	F86I	30	2.1±0.3	2680±230	373	−5730±805	−3.2	−4760
	F86P	30	2.3±0.3	9800±1300	102	−2571±188	9.8	−5542