Table 3. Thermodynamic parameters derived from calorimetric titrations of 2-oxoglutarate to Af-GlnK3 pre-incubated with MgATP.
| Wild-type | F86I | F86P | ||
| Temp. (°C) | 30 | 70 | 30 | 30 |
| Association constant (M−1) | ||||
| KA1 | 1.24E5±2.7E4 | 2.33E4±9.2E3 | 2.22E5±4.2E4 | 1.02E4±6.4E2 |
| KA2 | 2.62E3±9.1E2 | 7.67E2±2.9E2 | 1.40E4±3.5E3 | 4.09E3±2.6E2 |
| KA3 | 3.29E4±9.6E3 | 4.97E3±1.2E3 | 2.41E4±4.6E3 | 2.02E3±5.2E1 |
| Dissociation constant (µM) | ||||
| KD1 | 8.06 | 429.18 | 4.50 | 98.04 |
| KD2 | 381.68 | 1303.78 | 71.43 | 244.50 |
| KD3 | 30.40 | 201.21 | 41.49 | 495.05 |
| Free Enthalpy change (cal·mol−1) | ||||
| ΔH1 | 423±31 | −5367±956 | −852±60 | 2005±68 |
| ΔH2 | −2754±1170 | −46460±20100 | −2838±883 | −550±171 |
| ΔH3 | 7367±1120 | 6411±2140 | 9197±800 | 13220±147 |
| Entropy (cal·mol−1·K−1) | ||||
| ΔS1 | 24.7 | 4.4 | 21.7 | 24.9 |
| ΔS2 | 6.6 | −122.0 | 9.6 | 14.7 |
| ΔS3 | 45.0 | 35.6 | 50.4 | 58.7 |
| Gibbs Free Energy (cal·mol−1) | ||||
| ΔG1 | −7064.8 | −6868.4 | −7430.3 | −5543.4 |
| ΔG2 | −4739.6 | −4595.7 | −5748.2 | −5006.3 |
| ΔG3 | −6274.75 | −5805.1 | −6081.8 | −4574.9 |
Values are given for the first, second and third binding event.