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. 2011 Oct 19;6(10):e26466. doi: 10.1371/journal.pone.0026466

Figure 1. Characterization of the meri-1 promoter Sequence.

Figure 1

(A) Upregulation of meri-1 transcription induced by hd-siRNAs in CHO cells. CHO cells were transfected with indicated quantity of Nc siRNA_2. The meri-1 mRNA level was measured by qPCR, twelve hours after transfection. (B) Time course of hd-siRNA-stimulated transcription of meri-1. CHO cells were transfected with Nc siRNA_2. The meri-1 mRNA level was measured by qPCR. (C) Determination of the responsive ability of the 1.8 kb meri-1 promoter, Peri-1(−1654), to hd-siRNAs. CHO cells were co-transfected with GFP reporter construct containing the 1.8 kb meri-1 promoter and Nc siRNA_1 (siHBVP) (II), or 21-bp DNA control (I). (D) Response of the meri-1 promoter to enzymatically synthesized siRNA (Nc siRNA_1) and chemically synthesized siRNA (Nc siRNA_2). CHO cells were co-transfected with SEAP reporter construct containing the 1.8 kb meri-1 promoter and Nc siRNA_1 (panel 1), Nc siRNA_2 (panel 2), or the 21-bp DNA control (panel 3). (E) Response of the meri-1 promoter to hd-siRNA in the HEK 293 cells. (F) The necessity of liposome-mediated transfection process for the hd-siRNA-stimulated meri-1 promoter activity. CHO cells were transfected with GFP reporter construct containing the 1.8 kb meri-1 promoter. Four hours later, the medium was removed and the cells were washed three times by fresh medium. Then, Nc siRNA_1 was added to the medium without transfection agent (I). CHO cells were co-transfected with GFP reporter construct containing the 1.8 kb meri-1 promoter and Nc siRNA_1 was used as a control (II).