Table 2. Comparison of different filtering strategies for D. melanogaster.
Method | Data | Assembly size (Mb) | Assembled genome (Mb) | Accuracy | Recall | N50 | Reads (million) | |
Untrimmed | SRR063698 | full | 120.5 | 106.0 | 0.88 | 0.63 | 6,939 | 54.3 |
reduced | 113.4 | 84.4 | 0.74 | 0.50 | 1,717 | 13.7 | ||
SRR063699 | full | 108.1 | 77.4 | 0.72 | 0.45 | 1,278 | 26.8 | |
reduced | 63.6 | 41.2 | 0.65 | 0.24 | 411 | 6.7 | ||
ConDeTri | SRR063698 | full | 118.9 | 102,8 | 0.86 | 0.61 | 8,222 | 43.4 |
reduced | 103.6 | 65.9 | 0.64 | 0.39 | 1,007 | 11.0 | ||
SRR063699 | full | 77.5 | 59.0 | 0.76 | 0.35 | 508 | 10.0 | |
reduced | 23.4 | 18.0 | 0.77 | 0.11 | 234 | 2.5 | ||
SolexaQA | SRR063698 | full | 115.7 | 93.7 | 0.81 | 0.56 | 5,691 | 37.8 |
reduced | 93.4 | 51.5 | 0.55 | 0.31 | 677 | 9.6 | ||
SRR063699 | full | 60.5 | 40.2 | 0.66 | 0.24 | 408 | 8.7 | |
reduced | 16.7 | 11.6 | 0.70 | 0.07 | 203 | 2.2 | ||
Quake | SRR063698 | full | 114.3 | 81.6 | 0.71 | 0.48 | 21,615 | 49.6 |
reduced | 70.6 | 16.5 | 0.23 | 0.10 | 683 | 10.4 | ||
SRR063699 | full | 1.9 | 0.1 | 0.06 | 0.00 | 6,678 | 5.9 | |
reduced | 1.4 | 0.1 | 0.04 | 0.00 | 3,808 | 1.3 | ||
Bwa | SRR063698 | full | 119.4 | 99.1 | 0.83 | 0.59 | 13,953 | 46.2 |
reduced | 107.0 | 70.1 | 0.66 | 0.42 | 1,264 | 11.7 | ||
SRR063699 | full | 92.2 | 58.1 | 0.63 | 0.34 | 732 | 17.4 | |
reduced | 47.5 | 33.0 | 0.70 | 0.20 | 294 | 4.4 |
Assembly size, size of assembled D. melanogaster reference genome, recall, accuracy, N50 size and number of reads using the different data sets.