Figure 4. Genes near RMRP are not essential for cellular viability.

MEFs from E13.5 mice of either A. WT or B. RMRP+/− were transfected with siRNAs targeting Ccdc107 or E130. Three days later RNA was extracted from the cells and qRT-PCR was preformed using primers for RMRP, Ccdc107 or E130. C. The same cells as in A and B were plated (5000 cells/well) in a 96 well plate and 7 days post transfection cell number was assessed by Cell titer glow. Error bars represent SD of three replicas.