Table 1. Primer sequences used for RT–PCR.
| Gene | Accession No (reference) | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
|
Nanog |
NM_001129971.1 |
TGGAGTAACCCAACCTGGAG |
ATGATTTGCTGCTGGGTACC |
269 |
|
0ct4 |
NM_001113060.1 |
GTTTTGAGGCTTTGCAGCTC |
TCTCCAGGTTGCCTCTCACT |
183 |
|
cMyc |
NM_001005154.1 |
GGAAGGACTATCCCTCTGCC |
TCCAACTCTGGGATCTGGTC |
208 |
|
Klf4 |
NM_001031782.1 |
CAGCTTCAGCTATCCGATCC |
TGATGTCTGCCAGGTTGAAG |
128 |
|
Sox2 |
[21] |
GGCAGCTACAGCATGATGCAGGAGC |
CTGGTCATGGAGTTGTACTGCAGG |
131 |
|
Six3 |
[21] |
AGCGGACTCGGAGCCTGTTG |
AGCGCATGCCGCTCGGTCCA |
202 |
|
Otx2 |
XM_003121824.1 |
GCTGTGTGAATTGTGCGACT |
GGTGGAGTTCAAGGTTGCAT |
193 |
|
Mitf |
NM_001038001.1 |
GGGCCGCCTAAAGCGTGGT |
GGTCGCCAGGCTGGTTTGGAC |
198 |
|
Chx10 |
NM_182894.2 |
AGGGAGAACAGCATTGCGGTGC |
GCGCCTTGACCTAAGCCATGTCC |
193 |
|
Hes1 |
NM_001195231.1 |
CAGCCAGTGTCAACACGACAC |
TCGTTCATGCACTCACTGA |
307 |
|
HPRT |
NM_001032376.2 |
CCAGTCAACGGGCGATATAA |
CTTGACCAAGGAAAGCAAGG |
130 |
| PKC alpha |
XM_003131278.1 |
GACCATCCGCTCTACACTCAAC |
CCCAGTCCCAGATTTCTACAG |
104 |
| Calbindin |
NM_001130226.1 |
TCTGCTGGGGACAACTAAATTT |
CAGCCTACTCCGTTACAGTGCA |
93 |
| Rhodopsin | NM_214221.1 | TCCATCTACAACCCCGTCAT | CTGTCTTGGAAGTGGTGGTG | 127 |
Primers were either designed from sequences retrieved under the accession numbers shown, or taken from published studies.