Figure 4.

Control of LPS-induced cytokine secretion by Myd88, ERK1/2, and p38. Supernatants of the cultures used for macrophage spreading analysis were analyzed by ELISA for the presence of the cytokines TNF (A), IL-6 (B), IL-12p40 (C), and IL-10 (D). To normalize for differences between experiments in the absolute amount of cytokines produced, the maximum level of the LPS-treated WT or control macrophage was set to 100% (shown as ––). Shown are mean + SD values from two independent experiments.