FIGURE 7.

In vivo Nrd1 and Sen1 cross-linking downstream from protein-coding genes. The distribution of Nrd1 cross-linked RNA is shown in blue and Sen1 in green above the gene map. The position of poly(A) sites derived from RNA sequencing (Ozsolak et al. 2010) is shown in red. (A) Nrd1 and Sen1 cross-linking downstream from the TIS11 (CTH2) mRNA 3′ end. (B,C) Binding of Nrd1 and Sen1 in the 3′ UTR of the AHP1 and URA1 genes. (D) Binding of Nrd1 in the SNA3 3′ UTR. (E) Map of binding sites in the 3′ UTR. (F) Quantitative RT-PCR of RNA derived from wild-type or nrd1-102 cells grown at the nonpermissive temperature. The amplicon is located downstream from the SNA3 polyA site as indicated in D, and its relative abundance was normalized to the abundance of ACT1 in the same sample.