Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The specificity and sensitivity of one step SYBR green real-time RT-PCR. Plot of the amplification of a 10-fold serial dilution (1.31 × 107-1.31 × 102) of pDuCV-ORF-V1 to calculate the detection limit and sensitivity of real-time PCR by analyzing the fluorescence curve of the 191 bp DNA amplification product.
