Figure 2.

Real-time PCR standard curve generated from plasmid DNA amplification plot. Standard curve was plotted in the sample plasmid on the x-axis and cycle threshold (Ct) on the y-axis. The x-axis represents pDuCV-ORF-V1 in 10-fold dilutions and the y-axis the fluorescence data used for Ct determinations in dRn (baseline-corrected normalized fluorescence). The assays were linear range of pDuCV-ORF-V1 with R2 values (square of the correlation coefficient) of 0.993 and reaction efficiencies of 99%.