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. 2011 Oct 21;6(10):e26004. doi: 10.1371/journal.pone.0026004

Figure 6. Gal 3/LPS interaction enhances the fluorescence of FITC-LPS.

Figure 6

FITC-labeled E. coli LPS (10 µg/ml) was incubated with 4 µM Gal 3 or 2% serum for 2 hours, and then applied to a gel filtration column (Superdex TM 75 HR 10/30 column). FITC-LPS alone or Gal 3 alone were also analyzed. 0.5 ml fractions of FITC-LPS ± Gal 3 or ±2% serum were collected and further analyzed on a fluorescence microplate reader. The excitation and emission wavelengths were at 475 nm and 520 nm, respectively. Data represents the fluorescence of the main peak of FITC-LPS recovered in the void volume. ** p<0.01, ***p<0.001.