Table 2. Relative optical density (OD) for sOPN bands and OD-based concentration of sOPN in sputum.
Sputum | sOPN (µg/ml) | Relative OD | Relative OD | Relative OD | p-value |
All subjects (N = 33) | All subjects (N = 47) | Non-asthmatic Subjects (N = 27) | Asthmatic Subjects (N = 20) | ||
Polymeric sOPN (≥90 kD) (%) | 2.45±2.97 | 94.2±5.8 | 96.4±3.2 | 91.6±1.6 | 0.009 |
1.59 [0.58, 2.67] | 94.6 [92.7, 98.7] | 96.5 [94.6, 99.4] | 94.0 [89.0, 97.5] | ||
Monomeric sOPN (50–55 kD) (%) | 0.28±0.47 | 3.2±3.3 | 2.0±1.8 | 4.5±4.1 | 0.025 |
0.08 [0.00, 0.37] | 2.6 [0.7, 4.4] | 1.9 [0.0, 2.9] | 3.8 [1.6, 6.2] | ||
Cleaved sOPN (25 kD) (%) | 0.10±0.15 | 1.0±1.3 | 0.7±1.1 | 1.4±1.5 | 0.064 |
0.08 [0.00, 0.08] | 0.5 [0.0, 1.5] | 0.3 [0.0, 0.9] | 1.2 [0.3, 1.6] | ||
Cleaved sOPN (17 kD) (%) | 0.11±0.17 | 1.7±1.9 | 0.9±1.4 | 2.6±2.0 | 0.005 |
0.03 [0.01, 0.13] | 0.9 [0.3, 2.7] | 0.6 [0.0, 1.0] | 2.7 [0.6, 4.1] |
Values are expressed as mean ± SD and median [interquartile range]. Total concentration of sOPN was 3.03±3.41(2.25 [0.80, 3.08]) µg/ml in sputum samples. P-values are for comparisons (Mann-Whitney test) of relative OD of various bands between healthy and asthmatic subjects. Significant p-values are shown in bold.