Table 1. Primers used for amplifications of different DENV2 NS3 sequences for construction of the different recombinant plasmids.
| vectora | primers | Amplified regionb | Recombinant plasmid |
| pcTPA | 5′-GGGGGATATCGCTGGAGTATTGTGGG-3′c | protease (4522–5076) | pcTPANS3P |
| 5′-GGGTCTAGA TTACTTTCGAAAAATGTCATC-3′d | |||
| 5′-GGGGATATCAGAAAATTGACCATCATGG-3′c | helicase (5077–6375) | pcTPANS3H | |
| 5′-GGGGTCTAGA TTACTTTCTTCCAGCTGCA-3′d | |||
| 5′-GGGGGATATCGCTGGAGTATTGTGGG-3′c | NS3 (4522–6375) | pcTPANS3 | |
| 5′-GGGGTCTAGA TTACTTTCTTCCAGCTGCA-3′d | |||
| pcDNA3 | 5′-GGGGGATATC ATGGCTGGAGTATTGTGGG-3′c | protease (4522–5076) | pcNS3P |
| 5′-GGGTCTAGA TTACTTTCGAAAAATGTCATC-3′d | |||
| 5′-GGGGATATC ATGAGAAAATTGACCATCATGG-3′c | helicase (5077–6375) | pcNS3H | |
| 5′-GGGGTCTAGA TTACTTTCTTCCAGCTGCA-3′d | |||
| 5′-GGGGGATATC ATGGCTGGAGTATTGTGGG-3′c | NS3 (4522–6375) | pcNS3 | |
| 5′-GGGGTCTAGA TTACTTTCTTCCAGCTGCA-3′d |
a
Vectors used for cloning.
b
Genome coordinates [64].
c
Forward primer (start codon is marked in bold type and EcoRV restriction site is underlined).
d
Reverse primer (stop codon is marked in bold type and Xba I restriction site is underlined).