Figure 3.

Wnt4 promotes myogenic differentiation and induces fusion of C2C12 cells. (A) Troponin T expression after 3 day culture in proliferation or differentiation medium with recombinant adenoviruses expressing Wnt3a, Wnt4, Wnt5a or eGFP (control). Cell numbers are the means and standard deviations of three independent experiments performed in triplicate, *P < 0.01 vs. control. (B) Differentiated myotubes were evaluated by counting nuclei within fused multinucleated cells expressing troponin T. Four fields in triplicate cultures were used for counting fused cells cultured under a differentiation condition, *P < 0.04 vs. control.