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Effect of FH535 and K252a on C2C12 cell proliferation and differentiation. C2C12 cells were cultured with or without various FH535 and K252a concentrations followed by troponin T immunostaining. Total cell numbers are the counted DAPI-stained nuclei and the fusion index is the percentage of multinucleated cells among troponin T-positive cells. *P < 0.001 vs. absence of inhibitors.
