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Viscosity effects on the kinetic parameters of CPS catalyzed reactions with glycerol and PEG 8000 as cosolutes. Effects of glycerol are shown on kcat (A) and kcat/Km (B) with variable (2S,5S)-CMPr at constant ATP. The dashed lines have slopes of +1 and −1 in (A) and (B), respectively. Reported in the inset are the slopes of plots from either kcat°/kcat or (kcat/Km)°/(kcat/Km) versus the relative viscosity (η) of the solution.
