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. 2011 Oct 21;6(10):e26584. doi: 10.1371/journal.pone.0026584

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Furukawa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The traditional method requires individual construction of MAT a and MAT α haploid strains carrying different selection markers (linked to mutations or each strain). The diploid strains can be selected on plates lacking nutrients or containing antibiotics. (B) The new method proposed in this study generates homozygous diploids from a single haploid strain by subsequent use of the PGAL1-HO (galactose-inducible mating-type switch) and PSTE18-URA3 (counter selection marker for diploids) genes. The diploid strains are selected on plates containing 5-FOA, where non-mated haploid strains cannot grow.