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. 2011 Oct 21;6(10):e26696. doi: 10.1371/journal.pone.0026696

Figure 5. Hypersentivity to MMS, gamma-rays and UV irradiation of nhej, ssa and nhej ssa plants.

Figure 5

Before sowing, all seeds were surface-sterilized. (A) MMS hypersensitivity, 11 days post-germination. Seeds were sown on MS 0.5 agar 1% sucrose supplemented with MMS at various doses. (B) Gamma-irradiation hypersentivity, 7 days post-irradiation. After 48 h at 4°C in darkness, seeds were exposed to various doses of gamma-rays : 0, 100 and 200 grays before being sown on MS 0.5 agar. (C) UV hypersensitivity, 10 days post-irradiation. Seeds were sown in MS 0.5 agar. After 4 days of growth, the plantlets were exposed to UV-C, left in the dark for 3 days to avoid photoreactivation, and then exposed to light.