Figure 7. T-DNA insertion and expression in lig6 mutant.

(A) Position of the T-DNA insertion in AtLIG6. The structure of the AtLIG6 gene is represented by shaded boxes (exons) and thin lines (introns). The T-DNA insertion position is indicated. Each primer pair used to identify the mutants by PCR are indicated in black while primer pairs used for RT-PCR analyses are given in red; their localization is correct but not to of scale. (B) RT-PCR analysis of AtLIG6 transcripts in lig6-/- mutant plants. RNA, extracted from floral buds of wild-type or lig6 mutant plants was reverse-transcribed. Double-stranded cDNAs were amplified by RT-PCR, performed with three different primer pairs: 5′ or 3′ to the T-DNAand flanking the T-DNA. The position of each primer is given above (Figure 7A). The constitutive ACTIN gene was used as a control.