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. 2011 Oct 21;6(10):e26696. doi: 10.1371/journal.pone.0026696

Table 1. Sequence and use of primers in this study.

Name Gene DNA sequence (5′-3′) Use
87 Gabi T-DNA o8409 ATATTGACCATCATACTCATTGC genotyping
88 Salk T-DNA LBa1 TGGTTCACGTAGTGGGCCATCG genotyping
721 AtBRCA2a (At4g00020/10) GATTGTGCTCTGAATGCTAC genotyping
722 AtBRCA2a (At4g00020/10) CAATTTCTTTACCTTGAGGA genotyping
873 AtBRCA2a (At4g00020/10) ATGAGACCGATTGTGCTCTGAATGC RT-PCR
798 AtBRCA2a (At4g00020/10) CCAATTTCTTTACAGAAGCCTAGTCG RT-PCR
328 AtBRCA2b (At5g01630) GCTCTGAATATCAGTAAACCTGCT genotyping and RT-PCR
404 AtBRCA2b (At5g01630) TGTATCACACGATACAACAGACA genotyping
799 AtBRCA2b (At5g01630) TACAACAGACAAACCACTTGAAGCTTGCT RT-PCR
177 AtKU80 ((At1g48050) TGTCTTTTGCTTGTTGTGCAG genotyping
178 AtKU80 ((At1g48050) GCAGAAGGTGCAAGGTCAAG genotyping
456 AtKU80 ((At1g48050) ATGGCACGAAATCGGGAGGGTTTG RT-PCR
457 AtKU80 ((At1g48050) ACGATCAAGAAAGTCTCCAGCTAC RT-PCR
454 AtKU80 ((At1g48050) GAAGATTAAGGTGTGGGTTTATAAG RT-PCR
455 AtKU80 ((At1g48050) GTAAAACGAATCAGGAGTATCATCTC RT-PCR
458 AtKU80 ((At1g48050) CAAGGAGAATCCAAAGTTGAAGAAGG RT-PCR
459 AtKU80 ((At1g48050) CGTCTACTATATCACTGTCCGCTG RT-PCR
510 AtLIGASE6 (At1g66730) TCATTGCAGAATTGCTAAGGG genotyping
509 AtLIGASE6 (At1g66730) GAAGACGCAGACTTCAACCTG genotyping
637 AtLIGASE6 (At1g66730) AGAGCACGCTTGTTGGAGGG RT-PCR
638 AtLIGASE6 (At1g66730) TAAATTACGGGCCAATGTTCTAACAAG RT-PCR
686 AtLIGASE6 (At1g66730) GAGGGTGTTTCTGCTGCAGTAGTTGAGGCTTACAA RT-PCR
640 AtLIGASE6 (At1g66730) AAGAGCCAACAGCTGTTCTCCA RT-PCR
641 AtLIGASE6 (At1g66730) TTCATGGCTCAAGGTTAAGCGAGAT RT-PCR
642 AtLIGASE6 (At1g66730) GTTTGAGCATGAAACATCTCTGCGA RT-PCR
Act-464 AtACT2 (At3g18780) TGAGACCTTTAACTCTCCCG RT-PCR
Act-465 AtACT2 (At3g18780) GATGGCATGAGGAAGAGAGA RT-PCR
336 AtLIGIV (At5g57160) TTGCTGCTGAGGTATTGCAACGTAGAC RT-PCR
445 AtLIGIV (At5g57160) CCATCAAGGATACACTTGTCCACCAAT RT-PCR
452 AtERCC1 (At3g05210) CCCACAGTTCAAGCCAAACGCATC RT-PCR
453 AtERCC1 (At3g05210) ACATTCTGTCATGCTCCAGGCAC RT-PCR

See figures 1, 4 and 7 for the relative position of the primers.