Table 4. Allelic variation in 30 Physalis peruviana SSR loci.
Polymorphic loci | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR conditions | Alleles (pb) | Repeat type | Location | ||||
Primer [µM] | MgCl2 [mM] | °Tm | Expected size | Range size observed | ||||||
SSR1 | AGAGGACTCCATTTGTTTGCT | TGAGGGTGTTGGATGTTTTCT | 0,2 | 2 | 50 | 206 | 170 | 210 | AT | 3′ UTR |
SSR2 | CATTGGGTTTCGCATCCAT | AGACAAGCCTAGGGGAAAGG | 0,2 | 2 | 50 | 237 | 230 | 250 | AG | 3′ UTR |
SSR9 | TGCTCCGAGTTTTAGGGTTC | GCAGTTGGTAAAGTTGAGAGACG | 0,2 | 2 | 50 | 193 | 220 | 240 | AG | 5′ UTR |
SSR10 | GCTTCCTATTGTGTTGCCTGA | ACTTTGGGTTTCGGGAATTG | 0,2 | 2 | 50 | 185 | 170 | 190 | AT | 3′ UTR |
SSR11 | CAGCTGAAATAAGAGAGTGATTGG | CCCTCTTTTTCTCCTCCGAGT | 0,2 | 2 | 50 | 180 | 180 | 210 | AG | 3′ UTR |
SSR13 | GCGGAATCCATTGTTTTTCA | CCGATGAGATATAGTCACGCAAA | 0,2 | 2 | 50 | 190 | 160 | 210 | AC | 5′ UTR |
SSR14 | TGAAACCCATCTAGCTGAACG | TGGGTTGTTCCTTACAATCCAT | 0,2 | 1,5 | 50 | 204 | 200 | 220 | AT | 3′ UTR |
SSR15 | GCTTGTTGATCAGCTTTCTTTG | TGGATCATAACCTTGCTAATGC | 0,2 | 1,5 | 50 | 172 | 160 | 180 | AT | 3′ UTR |
SSR18 | CAGAGTGATTACCTTGGACGAA | TGTCCATTTTAGTCGCCAAT | 0,2 | 1,5 | 50 | 179 | 180 | 230 | AC | 3′ UTR |
SSR20 | GCACATCACATAAAGTATCTTTCTCA | TTGCCTGGTGTCTTGCTATG | 0,2 | 1,5 | 50 | 270 | 170 | 220 | AT | 3′ UTR |
SSR36 | ATGAACCACATGTCGGAGGA | GGGGATCCAAACGAAGTGTA | 0,2 | 1,5 | 52 | 211 | 170 | 240 | AG | 3′ UTR |
SSR37 | CCAACTGAATCAACACACAGC | CCACACTGAAAAAGGGATCTG | 0,3 | 2 | 50 | 212 | 260 | 330 | AG | 3′ UTR |
SSR54 | CGGCTGGTATGCTTACAAAGAT | GCACTTCCACTGTTTTTAACTTCC | 0,2 | 1,5 | 50 | 197 | 190 | 210 | AC | 3′ UTR |
SSR55 | CACCTACATAGGCAGCCAAAA | ATTTGTGGGCGGAGGAAG | 0,2 | 1,5 | 50 | 183 | 200 | 210 | AG | 5′ UTR |
SSR57 | AGTGAAAAGCAGCCCATTCT | GGCGAAGCTGAATTGAAAAA | 0,2 | 1,5 | 50 | 183 | 200 | 210 | AT | 3′ UTR |
SSR67 | GCTTCTGTTCCATTATTCACCA | GCAGTGTGGGATCAATCAAT | 0,2 | 1,5 | 50 | 207 | 180 | 240 | AG | 3′ UTR |
SSR68 | GAAGCAAACAACTACACCCAAA | AAGCCTCGGATTTCATAGCA | 0,2 | 1,5 | 50 | 187 | 160 | 220 | AG | 3′ UTR |
SSR72 | GTGCTCGCAGTTTCTTCAAA | CCGCCGTTACTTCCTAATCA | 0,2 | 1,5 | 50 | 158 | 130 | 170 | AG | 3′ UTR |
SSR77 | CATACCATAACTCCCCATCTCTC | TGCCGATTCTGATTTCTTCC | 0,2 | 1,5 | 50 | 216 | 170 | 200 | AT | 5′ UTR |
SSR92 | TGGTTTGAGGATCAAGAAAGAA | GTGGTATCAACGCAGAGTGG | 0,25 | 2,5 | 50 | 205 | 180 | 210 | AAG | 3′ UTR |
SSR107 | CATCCAACACCAGAAATACGC | TCCAACTTTATCATTTCTTCCAC | 0,2 | 1,5 | 50 | 206 | 220 | 250 | AAG | 5′ UTR |
SSR110 | CACCCATATCCCAATCTTCTTC | GGGTAATTTTCACGGGGAAT | 0,2 | 1,5 | 50 | 198 | 170 | 200 | CTT | 3′ UTR |
SSR112 | CTACGCCTACCACTTGCACA | CAGTGGAAGCCTCAAGATCC | 0,2 | 1,5 | 50 | 203 | 200 | 220 | TCT | 3′ UTR |
SSR118 | AATCAAGGGTCAGAAGAAATGG | GCAAGAATGGATGTGGGTGT | 0,2 | 1,5 | 50 | 180 | 130 | 180 | AAG | 5′ UTR |
SSR121 | AGCAACCTCCCAATCAGCTA | TGGTGAGTAAATGGGGGAAA | 0,2 | 1,5 | 50 | 189 | 170 | 190 | ATC | 3′ UTR |
SSR123 | TCAGTGGAGCGCGTATATCT | GCGATCTCACCAAACCTCTC | 0,2 | 1,5 | 50 | 216 | 190 | 210 | ATC | 5′ UTR |
SSR126 | TCCAAAAAGAAAACAAAAACACT | TTGAATGCATGTTTGATGGA | 0,2 | 1,5 | 50 | 202 | 190 | 200 | AGC | 5′ UTR |
SSR127 | TTGGTTTGGCATAACTGCAA | GGTTTGCAACTCTCATGCTG | 0,2 | 1,5 | 50 | 180 | 140 | 160 | AAT | 5′ UTR |
SSR138 | TCCGATCACTACTTCAGCACG | CAATTCGGGTTGTGAATCGGGT | 0,2 | 1,5 | 50 | 138 | 130 | 160 | AAT | 3′ UTR |
SSR146 | AGGCTAATGAGGACGAAGCA | GGTTGCATTACAAAGCACTGA | 0,2 | 1,5 | 50 | 187 | 160 | 210 | AAAAG | 3′ UTR |