Table 1. Comparison of the detected variations by 454 and Sanger Clone-based sequencing methods.

Method	Total number of variationsa		Average number of variations/per patient	Minor variationsb only detected by 454 or Cloning
	Total #c<20%	>20%		>10%<2%	2–10%
454 Sanger	1403411262	2772	20453	15464215	5289
	36321205	2427		642N/A	6

^a: a variation is defined in this study as ‘a change’ in a nucleotide sequence (either a clonal Sanger sequence or a 454 read) compared to the consensus population-based nucleotide sequence; b: a minor variation is defined as a nucleotide with an abundance less than 20% referred to the consensus population-based nucleotide sequence; c: # represents number.