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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Immunol. 2011 Sep 19;187(9):4667–4675. doi: 10.4049/jimmunol.1101949

Figure 1. Reduced thymic cellularity in mice with a Ras-binding mutant of p110γ.

Figure 1

(A) Flow cytometric analysis of CD4 and CD8 expression on T lymphocytes in the thymus from WT, p110γD/D and p110γD/D × p110δ−/− mice. The percentages of CD4+CD8+ double positive (DP) and CD4−CD8− double negative (DN) cells are shown. (B) Flow cytometric analysis of the DN thymocytes (identified as Thy1+ and [CD4/CD8/CD44/B220/CD11b/NK1.1/Gr1/Ter119/γδTCR]neg) and (C) the DN3/4 subsets. The percentages of CD25hiCD98lo (DN3a), CD25intCD98hi (DN3b) and CD25loCD98hi (DN4) are shown. (D) Thymic cellularity and the numbers of DN3a, DN3b, DN4 and DP thymocytes for each genotype. Graphs represent the mean and SEM (n ≥ 5).