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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Gene Ther. 2011 Jul 14;19(3):279–287. doi: 10.1038/gt.2011.107

Figure 6.

Figure 6

Comparison of Immunohistochemistry and Autoradiography for Identifying MV-NIS-Infected Regions of Tumors. Nine mice with MV-NIS-infected BxPC-3 flank tumors (6 intratumorally-injected and 3 intravenously-injected) and two control mice (no MV-NIS) were injected with 99mTc04 (37 MBq). Tumors were excised, frozen, sectioned, and mounted on slides. Images were taken from a representative tumor, removed 14 days after intravenous injection of MV-NIS. Upper panel, Autoradiographic image of a tumor section. Lower panel, The same section processed for immunohistochemical detection of the measles virus N protein. All MV-NIS–infected syncytia were positive by autoradiography. Arrows denote alignment of MV-NIS–infected regions. Note that areas of high cell density (lower right corner) correspond to low levels of background 99mTcO4 localization; areas of necrosis (holes) in the tumor have higher levels of background localization due to radionuclide pooling. MV-NIS denotes measles virus expressing sodium iodide symporter.