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. Author manuscript; available in PMC: 2011 Oct 22.
Published in final edited form as: J Comp Neurol. 2010 Jun 1;518(11):2090–2108. doi: 10.1002/cne.22323

Figure 4.

Figure 4

Restricted expression of hrGFP in leptin-responsive neurons as demonstrated by dual-label immunohistochemistry (case #4, LepRb-Cre-LacZ). Robust β-gal immunoreactivity (Alexa 594) is seen in leptin-responsive neurons distributed in the hypothalamus (A). By contrast, hrGFP-positive neurons (Alexa 488) are observed only in the DMH ipsilateral to the site of injection (B). High magnification photomicrographs reveal clusters of β-gal-positive neurons in the DMV (C). Dual-label labeling showing that hrGFP immunoreactivity coincided perfectly with β-gal (D). It shows that all hrGFP-positive neurons are also leptin-responsive neurons (arrows indicate colocalizations). However, some neurons positive for β-gal do not express hrGFP (arrowheads). Images have been converted to magenta-green. Scale bar in A applies to B, and scale bar in C applies to D. Abbreviations: 3v, third ventricle; Arc, arcuate nucleus of the hypothalamus; DMD, dorsal part of the dorsomedial nucleus of the hypothalamus; DMV, ventral part of the dorsomedial nucleus of the hypothalamus; f, fornix; VMH, ventromedial nucleus of the hypothalamus. Scale bars: A–B=200µm; C–D=50µm.

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