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. 2011 Nov;80(5):870–878. doi: 10.1124/mol.111.072363

Fig. 5.

Fig. 5.

Impact of SFN on proteasome-dependent loss of Ezh2 and Bmi-1. A, SCC-13 cells were treated with 20 μM SFN in the presence or absence of 0.5 μM lactacystin for 24 h. Total cell extracts were then prepared for immunodetection of Ezh2, Bmi-1, H3K27me3, and β-actin. B, SFN treatment increases general ubiquitination. Subconfluent SCC-13 cells were treated in the presence of 20 μM SFN for indicated times, and total cell extract was prepared and electrophoresed for detection of ubiquitin. β-Actin served as a loading control. C, SCC-13 cells were treated as above, and total extracts were immunoprecipitated with the indicated antibodies. The precipitates were then electrophoresed and immunoblotted to detect ubiquitin. Similar results were observed in each of three independent experiments.