Fig. 1.

CEBPD is down-regulated in prolactinomas. A, Principal Component Analysis (PCA) of microarray results derived from prolactinomas and normal rat pituitaries (Normal). B, Combined microarray data of human and rat prolactinomas identified CEBPD as a down-regulated gene in both species. C, CEBPD down-regulation in prolactinoma (PRLoma) was confirmed at the mRNA level by real-time PCR and protein levels were confirmed by Western blotting and immunofluorescence. The CEBPD (green) intensity in each PRL-positive (red) cell was quantified using ImageJ (mean ± sd; n = 3, *, P < 0.05).