Fig. 4.

CEBPD downstream signals. GH3 cells were transfected with CEBPD (GH3-CEBPD). Cells transfected with EV were used as control. Total RNA and protein were extracted 24 h after transfection and analyzed using real-time PCR or Western blot for c-Myc, cyclin D1, cyclin B1, cyclin B2, and survivin. B, GH3 cells transfected with either EV (control) or stably expressing CEBPD (GH3-CEBPD) were grown in media containing 10% serum (with serum) or 5% BSA (without serum). Proteins were analyzed by Western blotting for caspase 3 and cleaved caspase 3. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay was performed and apoptotic cells were counted. C, GH3 cells were transfected with CEBPD siRNA (GH3-siRNA). Cells transfected with scrambled siRNA were used as control. Total RNA and protein were extracted 24 h after transfection and analyzed by real-time PCR or Western blot for c-Myc, cyclin D1, cyclin B1, cyclin B2, and survivin (mean ± sd, n = 3; *, P < 0.05; **, P < 0.01).