Fig. 5.

Estrogen (E2) regulates CEBPD. A, GH3 cells were cultured in charcoal-stripped media, treated with E2 (10 nm) or estrogen receptor inhibitor ICI 182780 (ICI, 10 nm) for 48 h, and total RNA or protein extracted and analyzed by real-time PCR and Western blotting, respectively, for CEBPD. B, c-Myc, cyclins D1, B1, and B2, and survivin levels were measured in E2-induced rat prolactinoma (PRLoma). C, c-Myc, cyclins D1, B1, and B2, and survivin levels were measured in E2-treated GH3 cells (GH3–E2) and ICI-treated GH3 cells (GH3-ICI) (mean ± sd, n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001).