Fig. 5.

p62^P392L/CYLD shRNA stimulation of OCL differentiation in mouse bone marrow cultures. A, Mouse bone marrow-derived nonadherent cells were transduced with CYLD shRNA and coexpressed with p62^WT, p62^A381V, or p62^P392L. Cells were stimulated with RANKL (100 ng/ml) and M-CSF (10 ng/ml) for 5 d, and the TRAP (+) multinucleated OCL formed in these cultures were scored. B, Bone resorption activity of OCL on dentine. The percentage of resorbed area on dentine was quantified as described in Materials and Methods. The results represent quadruplicate cultures of three independent experiments (a and b, P < 0.05; compared with EV with and without RANKL stimulation, respectively).