Fig. 8.

A, Current-clamp recording from a quiescent GnRH-GFP neuron in a brain slice obtained from an adult male mouse with intact gonads showing that senktide (1 μm, 120 sec) had no effect on its membrane potential, whereas a subsequent application of Kiss1 (0.1 μm, 15 sec) produced a strong excitatory effect. B, In a spontaneously firing cell, NKB applied after administration of Kiss1 also had no effect. C and D, Current-clamp recordings from two Kiss1-sensitive GnRH-GFP neurons, recorded from brain slices obtained from GDX mice, showing that NKB had no discernible effect, regardless of whether it was administered before or after Kiss1.