Fig. 4.

Effect of estradiol on NPPC-mediated meiotic arrest in vitro. The proportion of oocytes having undergone GVB was determined at 4 and 24 h of culture. Bars indicate the mean ± sem of four independent experiments with at least 25 cumulus cell-enclosed oocytes assessed at each point in each experiment. Red bars, No estradiol; blue bars, 100 nm estradiol (E); solid bars, GVB assessed at 4 h; checkered bars, GVB assessed at 24 h. Percentages not connected by the same letter are significantly different (P < 0.05).