Fig. 8.
Expression the T-type Ca2+ channel CaV3.1 subtype in single Kiss1 neurons of female Kiss1-CreGFP OVX female mice. A, A representative gel illustrating the expression of CaV 3.1 and Kiss1 mRNA in Kiss1 neurons of the ARC. As a negative control, a cell reacted −RT did not express any of the transcripts. BH tissue was also included as a positive control (+, With RT) and negative control (−, Without RT). MM, Molecular markers. B, Summary bar graph of percentage expression of CaV3.1 in Kiss1 ARC neurons. An average of 20 cells/animal were harvested from six mice and were analyzed for CaV3.1 expression by scRT-PCR. Bar graphs represent the mean ± sem of the percentage of Kiss1 neurons expressing CaV3.1 per animal.