Fig. 6.
Increased body weight gain of pups nursed by REAf/+WAP-Cre (WC) females and its correlation with increased milk-related gene expression. A, The body weights of pups nursed by REAf/f or REAf/+WAP-Cre mice during postnatal development. The dam's genotype has a significant effect on the body weights of pups, with increased weight gain of the pups nursed by REAf/+ WAP-Cre dams (GLM, F1,472 = 156; P < 0.0001). GLM with repeated measures ANOVA. The data are mean ± sem (n = 39 or 40 per group). B, mRNA levels of REA, casein-β, casein-κ, α-lactalbumin, butyrophilin, and Elf-5 were examined by qRT-PCR at different stages of pregnancy (d 18) and lactation (d 2, 5, 9, and 14). The data are mean ± sd (n = 8 per group), and the expression was illustrated as relative expression normalized to 36B4 by wild-type P18. #, P < 0.05 vs. wild type.