Figure 3.

Antitumor effect of systemic administration of single adjuvants or their combinations. C57BL/6 mice (n = 6) were s.c. inoculated with 3 × 10⁴ TC-1 cells and immunized by a gene gun with 1 μg of pBSC/E7GGG.LAMP three and ten days later. The pBSC plasmid was used as a negative control. CpG-ODN (a), LMS (b), or their combinations (c) were i.p. injected at indicated doses on the days of DNA vaccination. The graphs (a), (b), and (c) were constructed from the results of the same experiment. No. of mice with a tumor/no. of mice in the group is indicated. Bars: ±SD; *P < 0.05.