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Assessment of Id2 expression in human colon tissues and cell lines. (a) Representative tissue sections of nonmalignant human colonic mucosa, primary colon adenocarcinoma, colon adenocarcinoma and liver metastasis were subjected to immunoperoxidase staining to detect expression of Id2 protein. (b) Western blot analysis of Id2 expression in six colon adenocarcinoma cell lines. Vinculin was used as an internal loading control. Id2, inhibitor of DNA-bind-2.
