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. Author manuscript; available in PMC: 2011 Oct 23.
Published in final edited form as: Oncogene. 2008 Sep 22;27(57):7192–7200. doi: 10.1038/onc.2008.356

Figure 3.

Figure 3

Effect of Id2 expression on apoptosis in HCT-116 cells. (a) Graphical representation of proportions of apoptotic control cells (Parental (Par) and scrambled-siRNA control (sh-Con)) and stable cell lines with reduced Id2 expression (sh-Id2C14 and sh-Id2C15) as determined by fluorescence-activated cell sorting analysis. (b) Western analysis of Bcl-2 family members Bim/Bod, Bcl-2, Bcl-xl, Bad and Bax from control cells (Par and sh-Con) and stable cell lines with reduced Id2 expression (sh-Id2C14 and sh-Id2C15). Vinculin levels were probed to ensure equal loading of all samples. (c) Western analysis of caspase-7/caspase-9 and PARP from control cells (Par and sh-Con) and stable cell lines with reduced Id2 expression (sh-Id2C14 and sh-Id2C15). Vinculin levels were probed to ensure equal loading of all samples. Id2, inhibitor of DNA-bind-2; PARP, poly (ADP-ribose) polymerase.