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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jun 13;1809(7):306–315. doi: 10.1016/j.bbagrm.2011.05.016

Fig. 1. DKK1 gene expression was induced by amino acid deprivation.

Fig. 1

a) Time course of the total amino acid deprivation treatment. SW480 was grown in complete medium (MEM) and amino acid deficiency medium (MEM-AA) for 0–24 h. Relative mRNA level was determined by quantitative real time RT-PCR. Data points were normalized by internal control L7a and to time zero. b) Transcription rate of DKK1 gene during amino acid deprivation. ChIP analysis was performed to examine Pol II binding at the coding region distal to the promoter. Data were presented as ratio to MEM. c) mRNA stability of DKK1 following amino acid deprivation. Each data point represents the mean ± S. D of three independent experiments. Asterisks (*) represent statistical significance compared to values in MEM (P < 0.05).