ALDH1L1 expression is selectively decreased in adult spinal cord astrocytes through transcriptional inactivation of the ALDH1L1 promoter A, Selective decrease of ALDH1L1 mRNA levels in spinal cord during postnatal development. N=5, one-way ANOVA with Bonferroni posthoc analysis, **P<0.01, *** P<0.001; mean ± SEM B, Selective decrease of ALDH1L1 protein levels in spinal cord during postnatal development. N=5 C, Loss of ALDH1L1 promoter activity indicated by eGFP fluorescence intensity in astrocytes of lumbar cord of BAC ALDH1L1 eGFP mice. Individual astrocytes are indicated by a white arrow. Scale bar, 20μm D, Quantitative measurement of the eGFP intensity decrease by FACS from spinal cord cell preparation of heterozygous BAC ALDH1L1 eGFP mice during postnatal development. MFI: mean fluorescence intensity. MFI was measured based upon at least 4000 cells/sample with a minimum of 3 mice for each experiment.