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. Author manuscript; available in PMC: 2012 Oct 4.
Published in final edited form as: Biochemistry. 2011 Sep 6;50(39):8497–8507. doi: 10.1021/bi2012355

Table 3.

Kinetics of Hydrophobic Pocket Mutants

OMPDC kcat (s−1) KM (µM) kcat/KM (M−1s−1) kex (s−1)
WT 4.0 ± 0.2 1.6 ± 0.1 2.0 × 106 0.015 ± 0.004
I96T 0.69 ± 0.05 2.3 ± 0.06 3.0 × 105 0.0047 ± 0.0004
I96S 0.011 ± 0.002 1.0 ± 0.04 1.0 × 104 0.006 ± 0.001
L123N 0.64 ± 0.01 3.5 ± 0.2 1.9 × 105 0.0007 ± 0.0001
L123S 0.36 ± 0.04 3.0 ± 0.5 1.2 × 105 0.00039 ± 0.00001
V155S 0.011 ± 0.003 4.7 ± 0.1 2.2 × 104 0.006 ± 0.003
V155D 5 ± 2 × 10−4 4.6 ± 0.5 4.6 × 102 0.057 ± 0.002
D70N 0.024 ± 0.05 6.0 ± 0.3 4.0 × 103 0.005 ± 0.002
D70N/I96S 6.0 (± 1.0) × 10−5
(5)a 		- - 0.002 ± 0.0003
(0.13)d
(22)a
D70N/L123S 9.3 (± 0.1) × 10−4
(0.1)b 		- - 0.00001 ± 0.000007
(1500)e
(1.7)b
D70N/V155S 1.8 ± (0.4) × 10−4
(1.5)c 		- - 0.0019 ± 0.0006
(0.13)f
(44)c
a

(D70N/I96S) / (D70N)(I96S)

b

(D70N/L123S) / (D70N)(L123S)

c

(D70N/V155S) / (D70N)(V155S)

d

(D70N/I96S) / (D70N)

e

(D70N/L123S) / (D70N)

f

(D70N/V155S) / (D70N)