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Kinetics of Ser 127 Mutants
| OMPDC | kcat (s−1) | KM (µM) | kcat/KM (M−1s−1) |
|---|---|---|---|
| WT | 4.6 ± 0.4 | 2.1 ± 0.1 | 2.2 (± 0.2) × 106 |
| S127A | 0.035 ± 0.002 | 96 ± 5 | 3.6 (± 0.3) × 102 |
| S127G | 0.26 ± 0.007 | 59 ± 5 | 4.3 (± 0.3) × 103 |
| S127P | - | - | 0.88 ± 0.16a |
Velocity is directly proportional to substrate concentration.
