Table 2.

qRT-PCR primers to quantitatively measure the mRNA levels of the PEPT1, PEPT2, ALAS1, ALAS2, ALAD, HMBS, UROS, UROD, ABCB6, CPOX, PPOX, ABCG2, FECH, HIF-1, and HO-1 genes

Gene	F/R	Primer Sequence	Position	Tm
PEPT1	Forward	CCCTGAAGTGAAGGTGTTTGAAGATA	1772–1797	60.6
NM_005073	Reverse	GAATTGGCCCCTGACATGAA	2168–2188	59.3
PEPT2	Forward	CTACCACAATATGCCCTGGTTACA	1894–1917	58.8
NM_001145998	Reverse	GCCACTGAACTGTGCCACAA	2045–2064	59.3
ALAS1	Forward	GGATTCGAAACAGCCGAGTG	1371–1390	59.3
NM_000688	Reverse	GAAGGTGATTGCTCCAAACTCAT	1542–1564	58.3
ALAS2	Forward	AAGATGTGAAGGCTTTCAAGACA	419–441	60
NM_000032	Reverse	GGAAAATGGCTTCCTTAGGC	470–489	60
ALAD	Forward	CCTCGGTTCCAACCAACTGAT	157–177	59.8
NM_000031	Reverse	GATAGGCTGTATGTCATCAGGAACA	319–343	58.2
HMBS	Forward	CAAGGACCAGGACATCTTGGAT	835–856	58.9
NM_000190	Reverse	CCAGACTCCTCCAGTCAGGTACA	984–1006	59.2
UROS	Forward	TCAGCACTGCCTCTTCTATTTCC	668–691	58.7
NM_000375	Reverse	CTGGGTGTGCAACTGTCTGATAC	761–789	58.3
UROD	Forward	CGGGAGTGTGTGGGGAA	982–998	57.2
NM_000374	Reverse	AAGCAGACGTGAGTGTTTATGCA	1178–1200	58.6
ABCB6	Forward	CAGAAGGGCCGTATTGAGTTTG	2033–2054	59.6
NM_005689	Reverse	ATTGTCGGCGATGGTGTCA	2308–2326	59.5
CPOX	Forward	GGCGGAGATGTTGCCTAAGAC	401–421	59.7
NM_000097	Reverse	AATGCTCACCCCAGCCTTTT	709–728	59.5
PPOX	Forward	CAGGAGTCCTGGGAATCGTGTA	1251–1272	59.9
NM_000309	Reverse	TGCCTAGCTGACTCTAGTTTTTGC	1509–1533	58.1
FECH	Forward	GGAAATCCATTGTTCTCTAAGGC	1252–1274	57
NM_001012515	Reverse	CTAAATAACACCCTCTCCACATCG	1462–1485	57.8
ABCG2	Forward	CTAAGCAGGGACGAACAATCATC	1188–1210	58.8
NM_004827	Reverse	TCCTGCTTGGAAGGCTCTATG	1447–1467	58.2
HIF-1	Forward	GGCGCGAACGACAAGAA	420–436	58
NM_001530	Reverse	C AAAACCATCCAAGGCTTTCA	682–702	58.7
HO-1	Forward	GCTCAAAAAGATTGCCCAGAA	518–538	58.1
NM_002133	Reverse	TCACATGGCATAAAGCCCTACA	926–947	59.1

Abbreviations: F/R, forward or reverse primers; T_m, melting temperature.