Fig. 5.

Effect of Bcl-2 overexpression on lanatoside C–induced cell death. U87 cells stably expressing a control vector, Bcl-2, or Bcl-xL were treated with different concentrations of lanatoside C (A), lanatoside C and tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) (B) or staurosporine (STS; positive control) (C). Twenty-four hours later, cell viability was assayed using CellTiter-Glo. The upper panel shows western blot analysis for overexpression of Bcl2 or Bcl-xl in these cells.