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. 2011 Oct 13;5:164. doi: 10.1186/1752-0509-5-164

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Copyright ©2011 Kippner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Simulated consequences of the redox effects of off-target variations in shRNA perturbation. Model predicted dynamics of intracellular H₂O₂accumulation (first column), glutathione redox potential (second column), thioredoxin redox potential (third column), and Pr-SSG accumulation (fourth column) after a 100 μM extracellular H₂O₂treatment. Solid lines represent the model-predicted behavior for the ideal KD cell lines with no off-target effects (w.t., Grx1, Trx1, G6PD), and dashed lines represent the model-predicted behavior for the experimentally characterized shRNA cell lines with qRT PCR-determined off-target effects (pLKO, Grx1*, Trx1*, G6PD*).