Figure 6.

G6PD off-target effect significantly alters intracellular redox capacity in knockdown cell lines. Model predicted dynamics of intracellular H₂O₂ accumulation (first column), glutathione redox potential (second column), thioredoxin redox potential (third column), and Pr-SSG accumulation (fourth column) in the Grx1 knockdown cell line after a 100 μM extracellular H₂O₂ treatment. Solid black lines represent the model-predicted behavior for the ideal knockdown cell lines with no off-target effects (Grx1), red dashed lines represent the model-predicted behavior for the experimentally characterized shRNA cell lines with all qRT PCR-determined off-target effects except those affecting the G6PD enzyme, and blue dotted lines represent the model-predicted behavior for Grx1 silencing only in the presence of G6PD off-target effects.