Figure 2.

Mapping fox sequencing reads against the canine RefSeq database: gene yield vs sequence depth. The yield of identifiable genes from fox transcriptome sequencing is plotted as a function of sequencing depth. Sets of reads, each set representing half a sequencing run (approximately 500,000 reads) were combined variously into 9 datasets each representing from half a run (9 datapoints, circled cluster at left extremity of plot) to 11 half-runs (1 datapoint, circled, rightmost extremity of plot). The vertical axis gives the percentage of genes in the canine RefSeq database hit by at last one read for each datapoint. Green diamonds = datapoints comprised of tame reads only; red squares = aggressive reads only; brown circles = datapoint combines tame and aggressive reads.