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. Author manuscript; available in PMC: 2012 Oct 21.
Published in final edited form as: ACS Chem Biol. 2011 Aug 23;6(10):1127–1135. doi: 10.1021/cb2001473

Figure 2.

Figure 2

F-C21 Labels PRMT1 in a Time and Concentration Dependent Manner. A. Time Course with 2 μM PRMT1 and 2 μM F-C21. B. Concentration dependence of F-C21with 2 μM PRMT1 and 30 minute reaction time. C. Concentration dependence of F-F21 with 2 μM PRMT1 and 30 minute reaction time. D. F-C21 limit of detection was determined to be 0.5 μM, which corresponds to 250 ng PRMT1. E. B-C21 limit of detection was determined to be 0.05 μM, which corresponds to 25 ng PRMT1.