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. Author manuscript; available in PMC: 2012 Oct 21.
Published in final edited form as: ACS Chem Biol. 2011 Aug 23;6(10):1127–1135. doi: 10.1021/cb2001473

Figure 4.

Figure 4

Labeling PRMT1 in MCF-7 Cell Lysates with B-C21. A. MCF-7 breast cancer whole cell extracts were labeled with increasing amounts of B-C21 for 30 minutes and subject to western blot analysis using a streptavidin-HRP conjugate. Proteins marked with (*) appear to be biotinylated proteins since they are present in the absence of B-C21. B. Selective Isolation of PRMT1 from MCF-7 Whole Cell Extracts with B-C21. MCF-7 WCE were incubated with the specified amount of B-C21 for 30 minutes, then bound to streptavidin agarose beads overnight at 4 °C. Beads were washed several times before bound proteins were eluted, separated by SDS-PAGE, and subject to western blot analysis with the appropriate antibody.